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Results: 1-24 |
Results: 24
REVERSED-FLOW AFFINITY ELUTION APPLIED TO THE PURIFICATION OF CARBOXYPEPTIDASE-Y
Authors: MORTENSEN UH STENNICKE HR BREDDAM K
Citation: Uh. Mortensen et al., REVERSED-FLOW AFFINITY ELUTION APPLIED TO THE PURIFICATION OF CARBOXYPEPTIDASE-Y, Analytical biochemistry, 258(2), 1998, pp. 236-239
PEPTIDE-SUBSTRATES DISSOLVED IN DIMETHYLFORMAMIDE MAY BE MODIFIED AT THE EPSILON-AMINO GROUP OF LYSYL RESIDUES, CAUSING ERRONEOUS KINETIC CHARACTERIZATION OF PROTEOLYTIC-ENZYMES
Authors: OLESEN K BREDDAM K
Citation: K. Olesen et K. Breddam, PEPTIDE-SUBSTRATES DISSOLVED IN DIMETHYLFORMAMIDE MAY BE MODIFIED AT THE EPSILON-AMINO GROUP OF LYSYL RESIDUES, CAUSING ERRONEOUS KINETIC CHARACTERIZATION OF PROTEOLYTIC-ENZYMES, Analytical biochemistry (Print), 262(1), 1998, pp. 88-89
THE SPECIFICITY OF CARBOXYPEPTIDASE-Y MAY BE ALTERED BY CHANGING THE HYDROPHOBICITY OF THE S-1' BINDING POCKET
Authors: SORENSEN SB BREDDAM K
Citation: Sb. Sorensen et K. Breddam, THE SPECIFICITY OF CARBOXYPEPTIDASE-Y MAY BE ALTERED BY CHANGING THE HYDROPHOBICITY OF THE S-1' BINDING POCKET, Protein science, 6(10), 1997, pp. 2227-2232
SUBSTRATES WITH CHARGED P-1 RESIDUES ARE EFFICIENTLY HYDROLYZED BY SERINE CARBOXYPEPTIDASES WHEN S-3-P-1 INTERACTIONS ARE FACILITATED
Authors: OLESEN K BREDDAM K
Citation: K. Olesen et K. Breddam, SUBSTRATES WITH CHARGED P-1 RESIDUES ARE EFFICIENTLY HYDROLYZED BY SERINE CARBOXYPEPTIDASES WHEN S-3-P-1 INTERACTIONS ARE FACILITATED, Biochemistry, 36(40), 1997, pp. 12235-12241
C-TERMINAL INCORPORATION OF FLUOROGENIC AND AFFINITY LABELS USING WILD-TYPE AND MUTAGENIZED CARBOXYPEPTIDASE-Y
Authors: STENNICKE HR OLESEN K SORENSEN SB BREDDAM K
Citation: Hr. Stennicke et al., C-TERMINAL INCORPORATION OF FLUOROGENIC AND AFFINITY LABELS USING WILD-TYPE AND MUTAGENIZED CARBOXYPEPTIDASE-Y, Analytical biochemistry, 248(1), 1997, pp. 141-148
PEPTIDE ALDEHYDE COMPLEXES WITH WHEAT SERINE CARBOXYPEPTIDASE-II - IMPLICATIONS FOR THE CATALYTIC MECHANISM AND SUBSTRATE-SPECIFICITY
Authors: BULLOCK TL BREDDAM K REMINGTON SJ
Citation: Tl. Bullock et al., PEPTIDE ALDEHYDE COMPLEXES WITH WHEAT SERINE CARBOXYPEPTIDASE-II - IMPLICATIONS FOR THE CATALYTIC MECHANISM AND SUBSTRATE-SPECIFICITY, Journal of Molecular Biology, 255(5), 1996, pp. 714-725
STUDIES ON THE HYDROLYTIC PROPERTIES OF (SERINE) CARBOXYPEPTIDASE-Y
Authors: STENNICKE HR MORTENSEN UH BREDDAM K
Citation: Hr. Stennicke et al., STUDIES ON THE HYDROLYTIC PROPERTIES OF (SERINE) CARBOXYPEPTIDASE-Y, Biochemistry, 35(22), 1996, pp. 7131-7141
INCREASE OF THE P-1 LYS LEU SUBSTRATE PREFERENCE OF CARBOXYPEPTIDASE-Y BY RATIONAL DESIGN BASED ON KNOWN PRIMARY AND TERTIARY STRUCTURES OFSERINE CARBOXYPEPTIDASES/
Authors: OLESEN K BREDDAM K
Citation: K. Olesen et K. Breddam, INCREASE OF THE P-1 LYS LEU SUBSTRATE PREFERENCE OF CARBOXYPEPTIDASE-Y BY RATIONAL DESIGN BASED ON KNOWN PRIMARY AND TERTIARY STRUCTURES OFSERINE CARBOXYPEPTIDASES/, Biochemistry, 34(48), 1995, pp. 15689-15699
SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE-Y FROM YEAST -THE SIGNIFICANCE OF THR60 AND MET398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS
Authors: SORENSEN SB RAASCHOUNIELSEN M MORTENSEN UH REMINGTON SJ BREDDAM K
Citation: Sb. Sorensen et al., SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE-Y FROM YEAST -THE SIGNIFICANCE OF THR60 AND MET398 IN HYDROLYSIS AND AMINOLYSIS REACTIONS, Journal of the American Chemical Society, 117(22), 1995, pp. 5944-5950
IMPROVEMENT OF THE APPLICABILITY OF CARBOXYPEPTIDASE-Y IN PEPTIDE-SYNTHESIS BY PROTEIN ENGINEERING
Authors: RAASCHOUNIELSEN M MORTENSEN UH OLESEN K BREDDAM K
Citation: M. Raaschounielsen et al., IMPROVEMENT OF THE APPLICABILITY OF CARBOXYPEPTIDASE-Y IN PEPTIDE-SYNTHESIS BY PROTEIN ENGINEERING, Peptide research, 7(3), 1994, pp. 132-135
A CONSERVED GLUTAMIC-ACID BRIDGE IN SERINE CARBOXYPEPTIDASES, BELONGING TO THE ALPHA BETA HYDROLASE FOLD, ACTS AS A PH-DEPENDENT PROTEIN-STABILIZING ELEMENT/
Authors: MORTENSEN UH BREDDAM K
Citation: Uh. Mortensen et K. Breddam, A CONSERVED GLUTAMIC-ACID BRIDGE IN SERINE CARBOXYPEPTIDASES, BELONGING TO THE ALPHA BETA HYDROLASE FOLD, ACTS AS A PH-DEPENDENT PROTEIN-STABILIZING ELEMENT/, Protein science, 3(5), 1994, pp. 838-842
CARBOXYPEPTIDASE Y MUTANTS WITH ALTERED SUBSTRATE SPECIFICITIES
Authors: OLESEN K BREDDAM K
Citation: K. Olesen et K. Breddam, CARBOXYPEPTIDASE Y MUTANTS WITH ALTERED SUBSTRATE SPECIFICITIES, Journal of cellular biochemistry, 1994, pp. 138-138
PEPTIDE-SYNTHESIS CATALYZED BY THE GLU ASP-SPECIFIC ENDOPEPTIDASE - INFLUENCE OF THE ESTER LEAVING GROUP OF THE ACYL DONOR ON YIELD AND CATALYTIC EFFICIENCY/
Authors: BONGERS J LIU W LAMBROS T BREDDAM K CAMPBELL RM FELIX AM HEIMER EP
Citation: J. Bongers et al., PEPTIDE-SYNTHESIS CATALYZED BY THE GLU ASP-SPECIFIC ENDOPEPTIDASE - INFLUENCE OF THE ESTER LEAVING GROUP OF THE ACYL DONOR ON YIELD AND CATALYTIC EFFICIENCY/, International journal of peptide & protein research, 44(2), 1994, pp. 123-129
EFFECTS OF INTRODUCED ASPARTIC AND GLUTAMIC-ACID RESIDUES ON THE P'(1)) SUBSTRATE-SPECIFICITY, PH-DEPENDENCE AND STABILITY OF CARBOXYPEPTIDASE-Y
Authors: STENNICKE HR MORTENSEN UH CHRISTENSEN U REMINGTON SJ BREDDAM K
Citation: Hr. Stennicke et al., EFFECTS OF INTRODUCED ASPARTIC AND GLUTAMIC-ACID RESIDUES ON THE P'(1)) SUBSTRATE-SPECIFICITY, PH-DEPENDENCE AND STABILITY OF CARBOXYPEPTIDASE-Y, Protein engineering, 7(7), 1994, pp. 911-916
PORTION-MIXING PEPTIDE LIBRARIES OF QUENCHED FLUOROGENIC SUBSTRATES FOR COMPLETE SUBSITE MAPPING OF ENDOPROTEASE SPECIFICITY
Authors: MELDAL M SVENDSEN I BREDDAM K AUZANNEAU FI
Citation: M. Meldal et al., PORTION-MIXING PEPTIDE LIBRARIES OF QUENCHED FLUOROGENIC SUBSTRATES FOR COMPLETE SUBSITE MAPPING OF ENDOPROTEASE SPECIFICITY, Proceedings of the National Academy of Sciences of the United Statesof America, 91(8), 1994, pp. 3314-3318
RECOGNITION OF C-TERMINAL AMIDE GROUPS BY (SERINE) CARBOXYPEPTIDASE-YINVESTIGATED BY SITE-DIRECTED MUTAGENESIS
Authors: MORTENSEN UH RAASCHOUNIELSEN M BREDDAM K
Citation: Uh. Mortensen et al., RECOGNITION OF C-TERMINAL AMIDE GROUPS BY (SERINE) CARBOXYPEPTIDASE-YINVESTIGATED BY SITE-DIRECTED MUTAGENESIS, The Journal of biological chemistry, 269(22), 1994, pp. 15528-15532
2.8-ANGSTROM STRUCTURE OF YEAST SERINE CARBOXYPEPTIDASE
Authors: ENDRIZZI JA BREDDAM K REMINGTON SJ
Citation: Ja. Endrizzi et al., 2.8-ANGSTROM STRUCTURE OF YEAST SERINE CARBOXYPEPTIDASE, Biochemistry, 33(37), 1994, pp. 11106-11120
THE ACTIVITY OF CARBOXYPEPTIDASE-Y TOWARD SUBSTRATES WITH BASIC P-1 AMINO-ACID-RESIDUES IS DRASTICALLY INCREASED BY MUTATIONAL REPLACEMENT OF LEUCINE-178
Authors: OLESEN K MORTENSEN UH AASMULOLSEN S KIELLANDBRANDT MC REMINGTON SJ BREDDAM K
Citation: K. Olesen et al., THE ACTIVITY OF CARBOXYPEPTIDASE-Y TOWARD SUBSTRATES WITH BASIC P-1 AMINO-ACID-RESIDUES IS DRASTICALLY INCREASED BY MUTATIONAL REPLACEMENT OF LEUCINE-178, Biochemistry, 33(37), 1994, pp. 11121-11126
SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE-Y - A HYDROGEN-BOND NETWORK STABILIZES THE TRANSITION-STATE BY INTERACTION WITH THE C-TERMINAL CARBOXYLATE GROUP OF THE SUBSTRATE
Authors: MORTENSEN UH REMINGTON SJ BREDDAM K
Citation: Uh. Mortensen et al., SITE-DIRECTED MUTAGENESIS ON (SERINE) CARBOXYPEPTIDASE-Y - A HYDROGEN-BOND NETWORK STABILIZES THE TRANSITION-STATE BY INTERACTION WITH THE C-TERMINAL CARBOXYLATE GROUP OF THE SUBSTRATE, Biochemistry, 33(2), 1994, pp. 508-517
MECHANISTIC STUDY ON CARBOXYPEPTIDASE Y-CATALYZED TRANSACYLATION REACTIONS - MUTATIONALLY ALTERED ENZYMES FOR PEPTIDE-SYNTHESIS
Authors: MORTENSEN UH STENNICKE HR RAASCHOUNIELSEN M BREDDAM K
Citation: Uh. Mortensen et al., MECHANISTIC STUDY ON CARBOXYPEPTIDASE Y-CATALYZED TRANSACYLATION REACTIONS - MUTATIONALLY ALTERED ENZYMES FOR PEPTIDE-SYNTHESIS, Journal of the American Chemical Society, 116(1), 1994, pp. 34-41
THE PRIMARY STRUCTURE OF CARBOXYPEPTIDASE-S1 FROM PENICILLIUM-JANTHINELLUM
Authors: SVENDSEN I HOFMANN T ENDRIZZI J REMINGTON SJ BREDDAM K
Citation: I. Svendsen et al., THE PRIMARY STRUCTURE OF CARBOXYPEPTIDASE-S1 FROM PENICILLIUM-JANTHINELLUM, FEBS letters, 333(1-2), 1993, pp. 39-43
A GLUTAMIC-ACID SPECIFIC SERINE-PROTEASE UTILIZES A NOVEL HISTIDINE TRIAD IN SUBSTRATE-BINDING
Authors: NIENABER VL BREDDAM K BIRKTOFT JJ
Citation: Vl. Nienaber et al., A GLUTAMIC-ACID SPECIFIC SERINE-PROTEASE UTILIZES A NOVEL HISTIDINE TRIAD IN SUBSTRATE-BINDING, Biochemistry, 32(43), 1993, pp. 11469-11475
MUTATIONAL REPLACEMENTS OF THE AMINO-ACID-RESIDUES FORMING THE HYDROPHOBIC S(4) BINDING POCKET OF SUBTILISIN-309 FROM BACILLUS-LENTUS
Authors: SORENSEN SB BECH LM MELDAL M BREDDAM K
Citation: Sb. Sorensen et al., MUTATIONAL REPLACEMENTS OF THE AMINO-ACID-RESIDUES FORMING THE HYDROPHOBIC S(4) BINDING POCKET OF SUBTILISIN-309 FROM BACILLUS-LENTUS, Biochemistry, 32(35), 1993, pp. 8994-8999
APPLICATION OF QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP MODELING TO THE EVALUATION OF THE CHANGES IN ENZYMATIC-ACTIVITY OF CARBOXYPEPTIDASE Y UPON CHEMICAL MODIFICATIONS
Authors: KANSTRUP A BREDDAM K BUCHARDT O
Citation: A. Kanstrup et al., APPLICATION OF QUANTITATIVE STRUCTURE-ACTIVITY RELATIONSHIP MODELING TO THE EVALUATION OF THE CHANGES IN ENZYMATIC-ACTIVITY OF CARBOXYPEPTIDASE Y UPON CHEMICAL MODIFICATIONS, Archives of biochemistry and biophysics, 304(2), 1993, pp. 332-337
Risultati: 1-24 |