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Results: 1-25 | 26-30
Results: 1-25/30
Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytictriad
Authors: Snijder, HJ Van Eerde, JH Kingma, RL Kalk, KH Dekker, N Egmond, MR Dijkstra, BW
Citation: Hj. Snijder et al., Structural investigations of the active-site mutant Asn156Ala of outer membrane phospholipase A: Function of the Asn-His interaction in the catalytictriad, PROTEIN SCI, 10(10), 2001, pp. 1962-1969
Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site
Authors: Vandeputte-Rutten, L Kramer, RA Kroon, J Dekker, N Egmond, MR Gros, P
Citation: L. Vandeputte-rutten et al., Crystal structure of the outer membrane protease OmpT from Escherichia coli suggests a novel catalytic site, EMBO J, 20(18), 2001, pp. 5033-5039
Cloning, purification and characterisation of Staphylococcus warneri lipase 2
Authors: van Kampen, MD Rosenstein, R Gotz, F Egmond, MR
Citation: Md. Van Kampen et al., Cloning, purification and characterisation of Staphylococcus warneri lipase 2, BBA-PROT ST, 1544(1-2), 2001, pp. 229-241
Structural investigations of calcium binding and its role in activity and activation of outer membrane phospholipase A from Escherichia coli
Authors: Snijder, HJ Kingma, RL Kalk, KH Dekker, N Egmond, MR Dijkstra, BW
Citation: Hj. Snijder et al., Structural investigations of calcium binding and its role in activity and activation of outer membrane phospholipase A from Escherichia coli, J MOL BIOL, 309(2), 2001, pp. 477-489
Bactericidal properties of group IIA and group V phospholipases A(2)
Authors: Gronroos, JO Laine, VJO Janssen, MJW Egmond, MR Nevalainen, TJ
Citation: Jo. Gronroos et al., Bactericidal properties of group IIA and group V phospholipases A(2), J IMMUNOL, 166(6), 2001, pp. 4029-4034
Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site
Authors: Kramer, RA Vandeputte-Rutten, L de Roon, GJ Gros, P Dekker, N Egmond, MR
Citation: Ra. Kramer et al., Identification of essential acidic residues of outer membrane protease OmpT supports a novel active site, FEBS LETTER, 505(3), 2001, pp. 426-430
Cloning, expression, purification and characterization of patatin, a novelphospholipase A
Authors: Hirschberg, HJHB Simons, JWFA Dekker, N Egmond, MR
Citation: Hjhb. Hirschberg et al., Cloning, expression, purification and characterization of patatin, a novelphospholipase A, EUR J BIOCH, 268(19), 2001, pp. 5037-5044
Substrate specificity of the integral membrane protease OmpT determined byspatially addressed peptide libraries
Authors: Dekker, N Cox, RC Kramer, RA Egmond, MR
Citation: N. Dekker et al., Substrate specificity of the integral membrane protease OmpT determined byspatially addressed peptide libraries, BIOCHEM, 40(6), 2001, pp. 1694-1701
Directed evolution: From a staphylococcal lipase to a phospholipase
Authors: van Kampen, MD Egmond, MR
Citation: Md. Van Kampen et Mr. Egmond, Directed evolution: From a staphylococcal lipase to a phospholipase, EUR J LIPID, 102(12), 2000, pp. 717-726
Activity and enantioselectivity of modified lipases in organic solvents
Authors: Overbeeke, PLA Koops, BC Verheij, HM Slotboom, AJ Egmond, MR Jongejan, JA Heijnen, JJ
Citation: Pla. Overbeeke et al., Activity and enantioselectivity of modified lipases in organic solvents, BIOCATAL B, 18(1), 2000, pp. 59-77
Fusarium solani pisi cutinase
Authors: Egmond, MR de Vlieg, J
Citation: Mr. Egmond et J. De Vlieg, Fusarium solani pisi cutinase, BIOCHIMIE, 82(11), 2000, pp. 1015-1021
Substitutions of surface amino acid residues of cutinase probed by aqueoustwo-phase partitioning
Authors: Berggren, K Egmond, MR Tjerneld, F
Citation: K. Berggren et al., Substitutions of surface amino acid residues of cutinase probed by aqueoustwo-phase partitioning, BBA-PROT ST, 1481(2), 2000, pp. 317-327
Identification of active site serine and histidine residues in Escherichiacoli outer membrane protease OmpT
Authors: Kramer, RA Dekker, N Egmond, MR
Citation: Ra. Kramer et al., Identification of active site serine and histidine residues in Escherichiacoli outer membrane protease OmpT, FEBS LETTER, 468(2-3), 2000, pp. 220-224
In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT
Authors: Kramer, RA Zandwijken, D Egmond, MR Dekker, N
Citation: Ra. Kramer et al., In vitro folding, purification and characterization of Escherichia coli outer membrane protease OmpT, EUR J BIOCH, 267(3), 2000, pp. 885-893
Unusual catalytic triad of Escherichia coli outer membrane phospholipase A
Authors: Kingma, RL Fragiathaki, M Snijder, HJ Dijkstra, BW Verheij, HM Dekker, N Egmond, MR
Citation: Rl. Kingma et al., Unusual catalytic triad of Escherichia coli outer membrane phospholipase A, BIOCHEM, 39(33), 2000, pp. 10017-10022
Enzymatic properties of rat group IIA and V phospholipases A(2) compared
Authors: Janssen, MJW Vermeulen, L Van der Helm, HA Aarsman, AJ Slotboom, AJ Egmond, MR
Citation: Mjw. Janssen et al., Enzymatic properties of rat group IIA and V phospholipases A(2) compared, BBA-MOL C B, 1440(1), 1999, pp. 59-72
Catalytic role of the active site histidine of porcine pancreatic phospholipase A2 probed by the variants H48Q, H48N and H48K
Authors: Janssen, MJW van de Wiel, WAEC Beiboer, SHW van Kampen, MD Verheij, HM Slotboom, AJ Egmond, MR
Citation: Mjw. Janssen et al., Catalytic role of the active site histidine of porcine pancreatic phospholipase A2 probed by the variants H48Q, H48N and H48K, PROTEIN ENG, 12(6), 1999, pp. 497-503
Activity and stability of chemically modified Candida antarctica lipase B adsorbed on solid supports
Authors: Koops, BC Papadimou, E Verheij, HM Slotboom, AJ Egmond, MR
Citation: Bc. Koops et al., Activity and stability of chemically modified Candida antarctica lipase B adsorbed on solid supports, APPL MICR B, 52(6), 1999, pp. 791-796
Effect of chemical modification on the activity of lipases in organic solvents
Authors: Koops, BC Verheij, HM Slotboom, AJ Egmond, MR
Citation: Bc. Koops et al., Effect of chemical modification on the activity of lipases in organic solvents, ENZYME MICR, 25(7), 1999, pp. 622-631
Structural evidence for dimerization-regulated activation of an integral membrane phospholipase
Authors: Snijder, HJ Ubarretxena-Belandia, I Blaauw, M Kalk, KH Verheij, HM Egmond, MR Dekker, N Dijkstra, BW
Citation: Hj. Snijder et al., Structural evidence for dimerization-regulated activation of an integral membrane phospholipase, NATURE, 401(6754), 1999, pp. 717-721
Introduction of a C-terminal aromatic sequence from snake venom phospholipases A(2) into the porcine pancreatic isozyme dramatically changes the interfacial kinetics
Authors: Janssen, MJW Burghout, PJ Verheij, HM Slotboom, AJ Egmond, MR
Citation: Mjw. Janssen et al., Introduction of a C-terminal aromatic sequence from snake venom phospholipases A(2) into the porcine pancreatic isozyme dramatically changes the interfacial kinetics, EUR J BIOCH, 263(3), 1999, pp. 782-788
Engineering the disulphide bond patterns of secretory phospholipases A2 into porcine pancreatic isozyme - The effects on folding, stability and enzymatic properties
Authors: Janssen, MJW Verheij, HM Slotboom, AJ Egmond, MR
Citation: Mjw. Janssen et al., Engineering the disulphide bond patterns of secretory phospholipases A2 into porcine pancreatic isozyme - The effects on folding, stability and enzymatic properties, EUR J BIOCH, 261(1), 1999, pp. 197-207
Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor
Authors: Ubarretxena-Belandia, I Cox, RC Dijkman, R Egmond, MR Verheij, HM Dekker, N
Citation: I. Ubarretxena-belandia et al., Half-of-the-sites reactivity of outer-membrane phospholipase A against an active-site-directed inhibitor, EUR J BIOCH, 260(3), 1999, pp. 794-800
Interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis
Authors: Flipsen, JAC van Schaick, MA Dijkman, R van der Hijden, HTWM Verheij, HM Egmond, MR
Citation: Jac. Flipsen et al., Interfacial binding of cutinase rather than its catalytic activity determines the steady state interfacial tension during oil drop lipid hydrolysis, CHEM PHYS L, 97(2), 1999, pp. 181-191
Modifying the substrate specificity of staphylococcal lipases
Authors: van Kampen, M Verheij, HM Egmond, MR
Citation: M. Van Kampen et al., Modifying the substrate specificity of staphylococcal lipases, BIOCHEM, 38(29), 1999, pp. 9524-9532
Risultati: 1-25 | 26-30