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Results: 1-11 |
Results: 11
Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention ofundesired multipoint adsorptions
Authors: Mateo, C Fernandez-Lorente, G Pessela, BCC Vian, A Carrascosa, AV Garcia, JL Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention ofundesired multipoint adsorptions, J CHROMAT A, 915(1-2), 2001, pp. 97-106
Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions
Authors: Fernandez-Lafuente, R Hernandez-Justiz, O Mateo, C Terreni, M Fernandez-Lorente, G Moreno, MA Alonso, J Garcia-Lopez, JL Guisan, JM
Citation: R. Fernandez-lafuente et al., Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions, BIOMACROMOL, 2(1), 2001, pp. 95-104
Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium
Authors: Fernandez-Lorente, G Fernandez-Lafuente, R Palomo, JM Mateo, C Bastida, A Coca, J Haramboure, T Hernandez-Justiz, O Terreni, M Guisan, JM
Citation: G. Fernandez-lorente et al., Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium, J MOL CAT B, 11(4-6), 2001, pp. 649-656
Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization withpolyethylenimine
Authors: Guisan, JM Fernandez-Lafuente, R Fernandez-Lorente, G Mateo, C Halling, PJ Kennedy, D Miyata, E Re, D
Citation: Jm. Guisan et al., Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization withpolyethylenimine, J MOL CAT B, 11(4-6), 2001, pp. 817-824
Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules
Authors: Abian, O Mateo, C Fernandez-Lorente, G Palomo, JM Fernandez-Lafuente, R Guisan, JM
Citation: O. Abian et al., Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules, BIOCATAL B, 19(5-6), 2001, pp. 489-503
Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase
Authors: Fernandez-Lorente, G Terreni, M Mateo, C Bastida, A Fernandez-Lafuente, R Dalmases, P Huguet, J Guisan, JM
Citation: G. Fernandez-lorente et al., Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase, ENZYME MICR, 28(4-5), 2001, pp. 389-396
One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports
Authors: Mateo, C Fernandez-Lorente, G Cortes, E Garcia, JL Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports, BIOTECH BIO, 76(3), 2001, pp. 269-276
Multifunctional epoxy supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage
Authors: Mateo, C Fernandez-Lorente, G Abian, O Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Multifunctional epoxy supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage, BIOMACROMOL, 1(4), 2000, pp. 739-745
Engineering of enzymes via immobilization and post-immobilization techniques: Preparation of enzyme derivatives with improved stability in organic media
Authors: Fernandez-Lorente, G Fernandez-Lafuente, R Armisen, P Sabuquillo, P Mateo, C Guisan, JM
Citation: G. Fernandez-lorente et al., Engineering of enzymes via immobilization and post-immobilization techniques: Preparation of enzyme derivatives with improved stability in organic media, METH TOOLS, 2000, pp. 36-51
Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques
Authors: Fernandez-Lafuente, R Rodriguez, V Mateo, C Fernandez-Lorente, G Arminsen, P Sabuquillo, P Guisan, JM
Citation: R. Fernandez-lafuente et al., Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques, J MOL CAT B, 7(1-4), 1999, pp. 173-179
Regioselective hydrolysis of peracetylated alpha-D-glucopyranose catalyzedby immobilized lipases in aqueous medium. A facile preparation of useful intermediates for oligosaccharide synthesis.
Authors: Bastida, A Fernandez-Lafuente, R Fernandez-Lorente, G Guisan, JM Pagani, G Terreni, M
Citation: A. Bastida et al., Regioselective hydrolysis of peracetylated alpha-D-glucopyranose catalyzedby immobilized lipases in aqueous medium. A facile preparation of useful intermediates for oligosaccharide synthesis., BIOORG MED, 9(4), 1999, pp. 633-636
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