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Results: 1-25 | 26-31
Results: 1-25/31
Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention ofundesired multipoint adsorptions
Authors: Mateo, C Fernandez-Lorente, G Pessela, BCC Vian, A Carrascosa, AV Garcia, JL Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Affinity chromatography of polyhistidine tagged enzymes - New dextran-coated immobilized metal ion affinity chromatography matrices for prevention ofundesired multipoint adsorptions, J CHROMAT A, 915(1-2), 2001, pp. 97-106
Structural and functional stabilization of L-asparaginase via multisubunitimmobilization onto highly activated supports
Authors: Balcao, VM Mateo, C Fernandez-Lafuente, R Malcata, FX Guisan, JM
Citation: Vm. Balcao et al., Structural and functional stabilization of L-asparaginase via multisubunitimmobilization onto highly activated supports, BIOTECH PR, 17(3), 2001, pp. 537-542
Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions
Authors: Fernandez-Lafuente, R Hernandez-Justiz, O Mateo, C Terreni, M Fernandez-Lorente, G Moreno, MA Alonso, J Garcia-Lopez, JL Guisan, JM
Citation: R. Fernandez-lafuente et al., Biotransformations catalyzed by multimeric enzymes: Stabilization of tetrameric ampicillin acylase permits the optimization of ampicillin synthesis under dissociation conditions, BIOMACROMOL, 2(1), 2001, pp. 95-104
Stabilization of a tetrameric enzyme (alpha-amino acid ester hydrolase from Acetobacter turbidans) enables a very improved performance of ampicillin synthesis
Authors: Fernandez-Lafuente, R Hernandez-Justiz, O Mateo, C Terreni, M Alonso, J Garcia-Lopez, JL Moreno, MA Guisan, JM
Citation: R. Fernandez-lafuente et al., Stabilization of a tetrameric enzyme (alpha-amino acid ester hydrolase from Acetobacter turbidans) enables a very improved performance of ampicillin synthesis, J MOL CAT B, 11(4-6), 2001, pp. 633-638
Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium
Authors: Fernandez-Lorente, G Fernandez-Lafuente, R Palomo, JM Mateo, C Bastida, A Coca, J Haramboure, T Hernandez-Justiz, O Terreni, M Guisan, JM
Citation: G. Fernandez-lorente et al., Biocatalyst engineering exerts a dramatic effect on selectivity of hydrolysis catalyzed by immobilized lipases in aqueous medium, J MOL CAT B, 11(4-6), 2001, pp. 649-656
Enantioselective enzymatic hydrolysis of racemic glycidyl esters by using immobilized porcine pancreas lipase with improved catalytic properties
Authors: Pregnolato, M Terreni, M de Fuentes, IE Leon, ARA Sabuquillo, P Fernandez-Lafuente, R Guisan, JM
Citation: M. Pregnolato et al., Enantioselective enzymatic hydrolysis of racemic glycidyl esters by using immobilized porcine pancreas lipase with improved catalytic properties, J MOL CAT B, 11(4-6), 2001, pp. 757-763
Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization withpolyethylenimine
Authors: Guisan, JM Fernandez-Lafuente, R Fernandez-Lorente, G Mateo, C Halling, PJ Kennedy, D Miyata, E Re, D
Citation: Jm. Guisan et al., Preparation of new lipases derivatives with high activity-stability in anhydrous media: adsorption on hydrophobic supports plus hydrophilization withpolyethylenimine, J MOL CAT B, 11(4-6), 2001, pp. 817-824
Electrostatic and covalent immobilisation of enzymes on ITQ-6 delaminated zeolitic materials
Authors: Corma, A Fornes, V Jorda, JL Rey, F Fernandez-Lafuente, R Guisan, JM Mateo, B
Citation: A. Corma et al., Electrostatic and covalent immobilisation of enzymes on ITQ-6 delaminated zeolitic materials, CHEM COMMUN, (5), 2001, pp. 419-420
Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules
Authors: Abian, O Mateo, C Fernandez-Lorente, G Palomo, JM Fernandez-Lafuente, R Guisan, JM
Citation: O. Abian et al., Stabilization of immobilized enzymes against water-soluble organic cosolvents and generation of hyper-hydrophilic micro-environments surrounding enzyme molecules, BIOCATAL B, 19(5-6), 2001, pp. 489-503
Modulation of penicillin acylase properties via immobilization techniques:One-pot chemoenzymatic synthesis of cephamandole from cephalosporin C
Authors: Terreni, M Pagani, G Ubiali, D Ferndandez-Lafuente, R Mateo, C Guisan, JM
Citation: M. Terreni et al., Modulation of penicillin acylase properties via immobilization techniques:One-pot chemoenzymatic synthesis of cephamandole from cephalosporin C, BIOORG MED, 11(18), 2001, pp. 2429-2432
Coimmobilization of L-asparaginase and glutamate dehydrogenases onto highly activated supports
Authors: Balcao, VM Mateo, C Fernandez-Lafuente, R Malcata, FX Guisan, JM
Citation: Vm. Balcao et al., Coimmobilization of L-asparaginase and glutamate dehydrogenases onto highly activated supports, ENZYME MICR, 28(7-8), 2001, pp. 696-704
Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase
Authors: Fernandez-Lorente, G Terreni, M Mateo, C Bastida, A Fernandez-Lafuente, R Dalmases, P Huguet, J Guisan, JM
Citation: G. Fernandez-lorente et al., Modulation of lipase properties in macro-aqueous systems by controlled enzyme immobilization: enantioselective hydrolysis of a chiral ester by immobilized Pseudomonas lipase, ENZYME MICR, 28(4-5), 2001, pp. 389-396
One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports
Authors: Mateo, C Fernandez-Lorente, G Cortes, E Garcia, JL Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., One-step purification, covalent immobilization, and additional stabilization of poly-His-tagged proteins using novel heterofunctional chelate-epoxy supports, BIOTECH BIO, 76(3), 2001, pp. 269-276
Multifunctional epoxy supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage
Authors: Mateo, C Fernandez-Lorente, G Abian, O Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Multifunctional epoxy supports: A new tool to improve the covalent immobilization of proteins. The promotion of physical adsorptions of proteins on the supports before their covalent linkage, BIOMACROMOL, 1(4), 2000, pp. 739-745
Essential role of the concentration of immobilized ligands in affinity chromatography: Purification of guanidinobenzoatase on an ionized ligand
Authors: Murza, A Fernandez-Lafuente, R Guisan, JM
Citation: A. Murza et al., Essential role of the concentration of immobilized ligands in affinity chromatography: Purification of guanidinobenzoatase on an ionized ligand, J CHROMAT B, 740(2), 2000, pp. 211-218
Stabilization of a beta-glucosidase from Aspergillus niger by binding to an amine agarose gel
Authors: Spagna, G Barbagallo, RN Pifferi, PG Blanco, RM Guisan, JM
Citation: G. Spagna et al., Stabilization of a beta-glucosidase from Aspergillus niger by binding to an amine agarose gel, J MOL CAT B, 11(2-3), 2000, pp. 63-69
A kinetic study of synthesis of amoxicillin using penicillin G acylase immobilized on agarose
Authors: Goncalves, LRB Fernandez-Lafuente, R Guisan, JM Giordano, RLC
Citation: Lrb. Goncalves et al., A kinetic study of synthesis of amoxicillin using penicillin G acylase immobilized on agarose, APPL BIOC B, 84-6, 2000, pp. 931-945
Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability
Authors: Fernandez-Lafuente, R Guisan, JM Ali, S Cowan, D
Citation: R. Fernandez-lafuente et al., Immobilization of functionally unstable catechol-2,3-dioxygenase greatly improves operational stability, ENZYME MICR, 26(8), 2000, pp. 568-573
Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment
Authors: Mateo, C Abian, O Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Increase in conformational stability of enzymes immobilized on epoxy-activated supports by favoring additional multipoint covalent attachment, ENZYME MICR, 26(7), 2000, pp. 509-515
Reversible enzyme immobilization via a very strong and nondistorting ionicadsorption on support-polyethylenimine composites
Authors: Mateo, C Abian, O Fernandez-Lafuente, R Guisan, JM
Citation: C. Mateo et al., Reversible enzyme immobilization via a very strong and nondistorting ionicadsorption on support-polyethylenimine composites, BIOTECH BIO, 68(1), 2000, pp. 98-105
Interaction of the antitumor drug 9-aminoacridine with guanidinobenzoatasestudied by spectroscopic methods: A possible tumor marker probe based on the fluorescence exciplex emission
Authors: Murza, A Sanchez-Cortes, S Garcia-Ramos, JV Guisan, JM Alfonso, C Rivas, G
Citation: A. Murza et al., Interaction of the antitumor drug 9-aminoacridine with guanidinobenzoatasestudied by spectroscopic methods: A possible tumor marker probe based on the fluorescence exciplex emission, BIOCHEM, 39(34), 2000, pp. 10557-10565
Engineering of enzymes via immobilization and post-immobilization techniques: Preparation of enzyme derivatives with improved stability in organic media
Authors: Fernandez-Lorente, G Fernandez-Lafuente, R Armisen, P Sabuquillo, P Mateo, C Guisan, JM
Citation: G. Fernandez-lorente et al., Engineering of enzymes via immobilization and post-immobilization techniques: Preparation of enzyme derivatives with improved stability in organic media, METH TOOLS, 2000, pp. 36-51
Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports
Authors: Armisen, P Mateo, C Cortes, E Barredo, JL Salto, F Diez, B Rodes, L Garcia, JL Fernandez-Lafuente, R Guisan, JM
Citation: P. Armisen et al., Selective adsorption of poly-His tagged glutaryl acylase on tailor-made metal chelate supports, J CHROMAT A, 848(1-2), 1999, pp. 61-70
Affinity chromatography of plasma proteins (guanidinobenzoatase): use of mimetic matrices and mimetic soluble ligands to prevent the binding of albumin on target affinity matrices
Authors: Murza, A Aguilar, AR Fernandez-Lafuente, R Guisan, JM
Citation: A. Murza et al., Affinity chromatography of plasma proteins (guanidinobenzoatase): use of mimetic matrices and mimetic soluble ligands to prevent the binding of albumin on target affinity matrices, J CHROMAT B, 732(1), 1999, pp. 165-172
Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques
Authors: Fernandez-Lafuente, R Rodriguez, V Mateo, C Fernandez-Lorente, G Arminsen, P Sabuquillo, P Guisan, JM
Citation: R. Fernandez-lafuente et al., Stabilization of enzymes (D-amino acid oxidase) against hydrogen peroxide via immobilization and post-immobilization techniques, J MOL CAT B, 7(1-4), 1999, pp. 173-179
Risultati: 1-25 | 26-31