J. Kopecny et B. Hodrova, THE EFFECT OF YELLOW AFFINITY SUBSTANCE ON CELLULASES OF RUMINOCOCCUS-FLAVEFACIENS, Letters in applied microbiology, 25(3), 1997, pp. 191-196
Cellulolytic cultures of Ruminococcus flavefaciens produced a yellow a
ffinity substance (YAS) with a strong affinity to microcrystalline cel
lulose (MC). YAS was bound to MC in the range of pH from 5 to 8 and at
temperatures from 10 degrees C to 60 degrees C. The positive effect o
f YAS on adsorption of ruminococcal cellulases was demonstrated by com
paring the adsorption behaviour of endoglucanases and cellobiohydrolas
es onto MC and YAS-treated MC. HPLC chromatography proved the presence
of two yellow compounds with affinity to cellulose as well as to rumi
nococcal cellulases. Both YAS compounds were sensitive to oxidation. T
he observed YAS properties showed a close relation to YS of Clostridiu
m thermocellum.