FLUORESCENCE AND TURBIDIMETRY STUDY OF COMPLEXATION OF HUMAN SERUM-ALBUMIN WITH POLYCATIONS

Complexation of poly(N-ethyl-4-vinylpyridinium bromide) (PEVP) and pol y(N-ethyl/cethyl-4-vinylpyridinium bromide) (PECVP) with human serum a lbumin (HSA) was studied by fluorescence spectroscopy and turbidimetri c titration in dilute aqueous solutions. Solubility of polycation-HSA complexes depends on the initial concentrations and ratios of the comp onents, pH, hydrophobic-hydrophilic balance of the macromolecules, and existence of low molecular salt (NaCl). A soluble interpolymer comple x which is formed at low concentrations of polycation is insoluble at high polycation concentrations. The average fluorescence quantum yield of HSA decreases as polycation binds to the protein. The maximum redu ction in emission intensity of HSA indicates the number of the HSA mol ecules bound per polymer chain. Quenching of HSA fluorescence by PECVP is decreased in the presence of NaCl at pH 7.0 but not at pH 4.3.