INTERACTION OF CHYMOTRYPSIN WITH CARBETOCIN -DEAMINO-1-MONOCARBA-2-O-METHYLTYROSINE]-OXYTOCIN)

The results of the present study describe the course of reaction and t he products following chymotrypsin treatment of the oxytocin analogue carbetocin deamino-1-monocarba-2-O-methyltyrosine]-oxytocin). The meta bolites were analyzed and identified through TLC, HPLC and mass spectr ometry. The main product emerging after treatment of carbetocin with c hymotrypsin is 9-desglycineamide carbetocin indicating preferential hy drolysis of the peptide bond between leucine at position 8 and carboxy terminal glycineamide. At the same time the stability of the bond betw een tyrosine at position 2 and isoleucine at position 3 appears signif icantly enhanced through the alkylation of the hydroxyl group of tyros ine.