EXPRESSION OF ESTROGEN-RECEPTOR-BETA IN RAT BONE

A never estrogen receptor, estrogen receptor beta (ER beta), has recen tly been cloned from a rat prostate cDNA library. In bone, which is an important target tissue of estrogen, ER alpha has been reported to be present preferentially in osteoblasts, but the mechanism of action of estrogen in bone is still not known. In the present study, we examine d expression of ER beta mRNA in bone. Expression of ER beta mRNA was e vident in primary osteoblastic cells isolated from 1-day-old rat calva ria and rat osteosarcoma cells (ROS 17/2.8), and its level was higher than that of ER alpha mRNA. When osteoblastic cells were cultured for 28 days to induce differentiation into mature osteoblasts capable of f orming bone nodules, ER beta mRNA was constantly and highly expressed during the entire culture period. In contrast, the level of ER alpha m RNA was very low at the beginning of culture and it gradually increase d during the differentiation of osteoblastic cells. Various tissues in cluding bone were isolated from 8-week-old rats of both sexes, and tot al RNA was extracted to compare the tissue distribution of expression levels of ER beta mRNA. In cancellous bone of the distal femoral metap hysis and lumbar vertebra, expression of ER beta mRNA was obvious, and its level was equivalent to those in the uterus and testis, but lower than those in the ovary and prostate. The level of ER beta mRNA in fe moral cortical bone was lower than that in cancellous bone. There was no appreciable differences between female and male rats in the distrib ution and expression levels of ER beta mRNA in bone. These results ind icate that ER beta mRNA is highly expressed in osteoblasts in rat bone , suggesting that there is a distinct mechanism of estrogen action med iated by ER beta in bone.