P. Amorim et al., NITROUS-OXIDE IMPAIRS ELECTROPHYSIOLOGIC RECOVERY AFTER SEVERE HYPOXIA IN RAT HIPPOCAMPAL SLICES, Anesthesiology, 87(3), 1997, pp. 642-651
Background Research has suggested that nitrous oxide may be harmful to
ischemic neurons; however, the evidence for this is equivocal. The au
thors used rat hippocampal slices to examine the effects of nitrous ox
ide on neuronal hypoxic damage. Methods: The evoked population spike C
PS) was recorded from hippocampal CA1 pyramidal cells before, during,
and after hypoxia. Control groups received nitrogen concentrations equ
al to nitrous oxide throughout the experiments. Biochemical measuremen
ts were made from dissected CA1 regions under experimental conditions
that matched the electrophysiology studies. Results: Recovery of the P
S after hypoxia was 18 +/- 7% in slices treated with 50% nitrous oxide
before and during 3.5 min of hypoxia; this compares with 41 +/- 9% (P
< 0.05) in nitrogen-treated slices. Slices treated with nitrous oxide
(95%) only during hypoxia (6 min) also demonstrated significantly les
s recovery of the PS than did slices treated with nitrogen. There was
no significant difference in recovery if nitrous oxide was discontinue
d after the hypoxic period. Adenosine triphosphate concentrations afte
r 3.5 min of hypoxia in slices treated with nitrous oxide decreased to
the same extent as in nitrogen-treated slices (47% vs. 50%). Calcium
influx increased during 10 min of hypoxia in untreated slices, but nit
rous oxide did not significantly increase calcium influx during hypoxi
a. The sodium concentrations increased and potassium concentrations de
creased during hypoxia; nitrous oxide did not significantly alter thes
e changes. Conclusions: Nitrous oxide impaired electrophysiologic reco
very of hippocampal slices after severe hypoxia. Nitrous oxide did not
cause significant changes in the biochemical parameters examined.