SIMULTANEOUS DETERMINATION OF MEBENDAZOLE AND PYRANTEL PAMOATE FROM TABLETS BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY REVERSE-PHASE (RP-HPLC)

A new, simple, precise, rapid and stability indicating RP-HPLC method has been developed for the simultaneous determination of mebendazole a nd pyrantel pamoate from tablets. Chromatography was carried out on a Shodex C8 column using a mixture of 0.05 M monobasic sodium phosphate: acetonitrile: triethylamine (60:40:1.5, v/v), adjusted to pH 6.8 with phosphoric acid. Detection was carried out at 290 nm using a UV detec tor. Retention times for pamoic acid, pyrantel base and mebendazole we re 3.61, 6.41 and 12.81 min, respectively. Linearity was obtained in t he concentration range of 20-160 mu g and 30-240 mu g for pyrantel pam oate and mebendazole, respectively. The method after its application f or the assay of pyrantel pamoate and mebendazole from tablets, was sta tistically evaluated for its accuracy and precision. (C) 1997 Elsevier Science B.V.