SEPARATION OF 15 NONSTEROIDAL ANTIINFLAMMATORY DRUGS USING MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

Micellar electrokinetic capillary chromatography with sodium dodecylsu lfate as anionic surfactant was used to separate simultaneously fiftee n non-steroidal anti-inflammatory drugs. UV detection was performed at 254 nm. The electroosmotic flow was carefully adjusted for optimal se paration. Resolution of the drugs was obtained using a buffer containi ng 40 mmol 1(-1) NaH2PO4, 0.103 mol 1(-1) sodium dodecylsulfate and 3% (v/v) methanol, adjusted to pH 8 with sodium hydroxide. Lack of inter ference was checked with a number of drugs and metabolites. Between-da y coefficients of variation ranged between 2 to 10%.