CAPILLARY ZONE ELECTROPHORESIS IN POLYMER NETWORKS OF POLYMERASE CHAIN REACTION-AMPLIFIED OLIGONUCLEOTIDES - THE CASE OF CONGENITAL ADRENAL-HYPERPLASIA

The use of capillary zone electrophoresis (CZE) in polymer networks fo r the analysis of an 8 bp (base pair) deletion in congenital adrenal h yperplasia was investigated. Separations were performed in Tris-borate -EDTA buffer (pH 8.3) containing 6% liquid linear polyacrylamide as a sieving dynamic matrix and 10 mu M ethidium bromide for improving DNA fragment separation, Easy analysis and detection of the 127 and 135 bp amplified fragments was accomplished. The capillary column can be use d for >50 analyses before degradation and loss of resolution. The resu lts are comparable to those obtained by gel-slab zone electrophoresis in a 12%T, 4%C polyacrylamide matrix. The sensitivity, by simple UV ab sorption at 254 nm, is similar to that obtained in gel slabs by dye in tercalation staining.