Aj. Harker et al., HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHIC ASSAY FOR 2'-DEOXY-3'-THIACYTIDINE IN HUMAN SERUM, Journal of chromatography B. Biomedical applications, 657(1), 1994, pp. 227-232
Citations number
4
Categorie Soggetti
Chemistry Analytical
Journal title
Journal of chromatography B. Biomedical applications
A high-performance liquid chromatographic method for the determination
of 2'-deoxy-3'-thiacytidine (3TC), a novel dideoxy-nucleoside analogu
e, in human serum is described. 3TC was extracted from serum samples u
sing Bond Elut Certify solid-phase extraction cartridges prior to reve
rsed-phase chromatography with UV detection. The method has been shown
to be valid over the concentration range 10-5000 ng/ml using a 1-ml s
ample volume, both before and after heat treatment of the samples at 6
0 degrees C for 3 h. The method has been automated using a Zymark robo
t and used in the analysis of serum samples from HIV positive patients
.