UNITED-ARAB-EMIRATES POPULATION-DATA ON 3 DNA TETRAMERIC SHORT TANDEMREPEAT LOCI - HUMTHO1, TPOX AND CSF1PO - DERIVED USING MULTIPLEX POLYMERASE CHAIN-REACTION AND MANUAL TYPING

Allele and genotype frequencies for three tetrameric short tandem repe at (STR) loci: HUMTH01, TPOX, and CSF1PO, were determined in a United Arab Emirates (UAE) national population sample (n = 119). The loci wer e amplified simultaneously and the PCR products were separated in poly acrylamide DNA sequencing gels. Detection of the DNA fragments was acc omplished using silver staining. Allele designations were determined b y comparison to an allelic ladder. One allele at each locus was sequen ced to confirm the nature of the repeats and their number. Alleles at the HUMTHO1 and TPOX loci were distributed bimodally, while CSF1PO sho wed unimodal distribution. The observed heterozygosities were 76% for HUMTHO1, 64% for TPOX, and 71% for CSF1PO. No deviation from the Hardy -Weinberg expectations was observed in the genotype distribution for t he loci TPOX and CSF1PO, but the HUMTHO1 locus did depart from HWE bas ed on the likelihood ratio and the exact test. No correlation was dete cted between the alleles at any of the three pairs of loci. The alleli c frequency data of these three loci in the UAE population sample can thus be used in human identity testing.