Jc. Castelli et al., A STUDY OF THE INTERFERON ANTIVIRAL MECHANISM - APOPTOSIS ACTIVATION BY THE 2-5A SYSTEM, The Journal of experimental medicine, 186(6), 1997, pp. 967-972
The 2-5A system contributes to the antiviral effect of interferons thr
ough the synthesis of 2-5A and its activation of the ribonuclease, RNa
se L. RNase L degrades viral and cellular RNA after activation by uniq
ue, 2'-5' phosphodiester-linked, oligoadenylates [2-5A, (pp)p5'A2'(P5'
A2')](n), n greater than or equal to 2. Because both the 2-5A system a
nd apoptosis can serve as viral defense mechanisms and RNA degradation
occurs during both processes, we investigated the potential role of R
Nase L in apoptosis. Overexpression of human RNase L by an inducible p
romoter in NIH3T3 fibroblasts decreased cell viability and triggered a
poptosis. Activation of endogenous RNase L, ape specifically with 2-5A
or with dsRNA, induced apoptosis. Inhibition of RNase L with a domina
nt negative mutant suppressed poly (I)poly (C)-induced apoptosis in in
terferon-primed fibroblasts. Moreover, inhibition of RNase L suppresse
d apoptosis induced by poliovirus. Thus, increased RNase L levels indu
ced apoptosis and inhibition of RNase L activity blocked viral-induced
apoptosis. Apoptosis may be one of the antiviral mechanisms regulated
by the 2-5A system.