DETERMINATION OF INDOLE-3-ACETIC-ACID IN PLANT-TISSUES BY CAPILLARY ELECTROPHORESIS

A capillary electrophoresis method with direct fluorimetric detection was developed for the determination of the plant growth regulator indo le-3-acetic acid (IAA) in plant tissues of Zea mays, Ricinus communis and Kalanchoe daigremontiana. Extraction of this metabolite from diffe rent plant organs, leaves and stem was performed with methanol, follow ed by a solid-phase extraction procedure. The subsequent capillary ele ctrophoresis step with a phosphate buffer and fluorimetric detection p rovides sufficient sensitivity to determine IAA in these plant samples . (C) 1997 Elsevier Science B.V.