UTILITY OF HBME-1 IMMUNOSTAINING IN SEROUS EFFUSIONS

Citation
V. Ascoli et al., UTILITY OF HBME-1 IMMUNOSTAINING IN SEROUS EFFUSIONS, Cytopathology, 8(5), 1997, pp. 328-335
Citations number
20
Categorie Soggetti
Pathology,"Cell Biology
Journal title
ISSN journal
09565507
Volume
8
Issue
5
Year of publication
1997
Pages
328 - 335
Database
ISI
SICI code
0956-5507(1997)8:5<328:UOHIIS>2.0.ZU;2-F
Abstract
HBME-1 is an anti-mesothelial cell monoclonal antibody derived from hu man mesothelioma cells, We investigated 227 body cavity effusions to t est its utility in differentiating mesothelioma from adenocarcinoma. H BME-1 outlined cell membranes in non-neoplastic mesothelial cells. Thi ck surface staining was observed on all mesotheliomas. HBME-1 reactivi ty was also detected in 24% of metastatic carcinomatous effusions, Mos t ovarian carcinomas (83%) reacted with this antibody, showing surface staining, Cytoplasmic HBME-1 immunoreactivity was observed in a small proportion of non-ovarian adenocarcinomas (14%). Despite its limited specificity, HBME-1 might be added to the battery of other markers of epithelial and/or mesothelial differentiation to be used in cases of s uspected mesothelioma, Evaluation of suspicious cells should include c areful study of the pattern of immunostaining.