GROWTH STAGE-DEPENDENT EFFECTS OF ELECTROMAGNETIC-FIELDS ON DNA-SYNTHESIS OF JURKAT CELLS

A 1.8 mT, bone healing, electromagnetic field (EMF) and power frequenc y EMFs of 0.1 and 0.4 mT significantly inhibit DNA synthesis in otherw ise unstimulated Jurkat (E 6.1) cells, Inhibition is generally most pr ominent in cells from mid log phase growth. In complete medium the bon e healing EMF inhibits [H-3] thymidine uptake of the latter cells by a lmost 50% vs. 20-25% inhibition by 60 Hz fields, Cells in conditioned medium are even more sensitive to EMFs with inhibition up to ca. 60%, The effects of the 0.1 and 0.4 mT poser frequency EMFs were very simil ar suggesting saturation at 0.1 mT or lower. (C) 1997 Federation of Eu ropean Biochemical Societies.