Xw. Li et al., EXPRESSION OF ICAM-1 IN IMPLANTED PRIMARY AND METASTATIC SQUAMOUS-CELL CARCINOMAS IN RATS, Journal of oral pathology & medicine, 26(8), 1997, pp. 371-376
FF6 tumor cells are dervied from a spontaneous rat squamous cell carci
noma (SCC) which originally arose in the facial skin of a DA rat. In t
his study, FF6 tumor cells were implanted into rat oral mucosa to esta
blish an ex vivo metastatic model. We analyzed the expression of inter
cellular cell adhesion molecule-1 (ICAM-1) in the implanted primary an
d metastatic FF6 tumors by immunostaining with a monoclonal antibody (
mAb) against ICAM-1. The implanted primary FF6 cells showed strong exp
ression of ICAM-1, whereas the tumor cells of metastatic lesions showe
d weak or negative expression of ICAM-1. By immunostaining with mAb OX
6, a number of MHC class II-positive macro phages were detected in tum
or mesenchyme and surrounding the metastatic foci. These results sugge
sted that the local immune reaction in the lymph node influenced the e
xpression of ICAM-1 on tumor cells, and that MHC class II-positive mac
rophages may play a role in transplanted tumor growth and metastases.