REGULATION OF INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 AND PROGESTERONE SECRETION FROM HUMAN GRANULOSA-LUTEAL CELLS - EFFECTS OF OCTREOTIDE AND INSULIN
N. Holst et al., REGULATION OF INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 AND PROGESTERONE SECRETION FROM HUMAN GRANULOSA-LUTEAL CELLS - EFFECTS OF OCTREOTIDE AND INSULIN, Fertility and sterility, 68(3), 1997, pp. 478-482
Objective: To examine the effects of the synthetic somatostatin-analog
ue octreotide and human recombinant insulin on the release of insulin-
like growth factor binding protein-1 (IGFBP-1) and P from human granul
osa-luteal cells. Design: Primary culture of human granulosa-luteal ce
lls. Setting: Academic research laboratory. Patient(s): Women undergoi
ng oocyte retrieval for NF-ET because of tubal infertility. Interventi
on(s): Octreotide or insulin mere added to the cultures; sampling of c
ulture medium was performed after 48 hours, Main Outcome Measure(s): I
nsulin-like growth factor binding protein-1 and P. Result(s): Octreoti
de significantly inhibited IGFBP-1 (58.8% compared with controls) and
P release (65.1% compared with controls). Insulin abolished IGFBP-1 re
lease while stimulating P release (200.7% compared with controls). The
re was a significant and positive correlation between IGFBP-1 and P le
vels. Conclusion(s): Octreotide and insulin have a significant effect
on human granulosa-luteal cell function in terms of IGFBP-1 and P rele
ase. Our results suggest a local ovarian mechanism for the recently ob
served effects of octreotide in the treatment of women with polycystic
ovary syndrome. (C) 1997 by American Society for Reproductive Medicin
e.