REGULATION OF INSULIN-LIKE GROWTH FACTOR-BINDING PROTEIN-1 AND PROGESTERONE SECRETION FROM HUMAN GRANULOSA-LUTEAL CELLS - EFFECTS OF OCTREOTIDE AND INSULIN

Objective: To examine the effects of the synthetic somatostatin-analog ue octreotide and human recombinant insulin on the release of insulin- like growth factor binding protein-1 (IGFBP-1) and P from human granul osa-luteal cells. Design: Primary culture of human granulosa-luteal ce lls. Setting: Academic research laboratory. Patient(s): Women undergoi ng oocyte retrieval for NF-ET because of tubal infertility. Interventi on(s): Octreotide or insulin mere added to the cultures; sampling of c ulture medium was performed after 48 hours, Main Outcome Measure(s): I nsulin-like growth factor binding protein-1 and P. Result(s): Octreoti de significantly inhibited IGFBP-1 (58.8% compared with controls) and P release (65.1% compared with controls). Insulin abolished IGFBP-1 re lease while stimulating P release (200.7% compared with controls). The re was a significant and positive correlation between IGFBP-1 and P le vels. Conclusion(s): Octreotide and insulin have a significant effect on human granulosa-luteal cell function in terms of IGFBP-1 and P rele ase. Our results suggest a local ovarian mechanism for the recently ob served effects of octreotide in the treatment of women with polycystic ovary syndrome. (C) 1997 by American Society for Reproductive Medicin e.