R. Sachdev et al., DETRIMENTAL EFFECT OF HYDROSALPINX FLUID ON THE DEVELOPMENT AND BLASTULATION OF MOUSE EMBRYOS IN-VITRO, Fertility and sterility, 68(3), 1997, pp. 531-533
Objective: To study the effects of human hydrosalpinx fluid on mouse e
mbryo blastulation rate. Design: Comparison of mouse embryo blastulati
on rate in media containing increasing concentrations of hydrosalpinx
fluid. Setting: Tertiary care center. Patient(s): Women undergoing lap
aroscopic evaluation or treatment for infertility noted to have hydros
alpinx or paratubal cyst. Intervention(s): Exposure of mouse embryos t
o hydrosalpinx or paratubal cyst fluid collected during laparoscopy. M
ain Outcome Measure(s): Blastulation rate of mouse embryos. Result(s):
Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydro
salpinx fluid concentrations demonstrated significantly lower blastula
tion rate at each level compared with the controls. Conclusion(s): Hyd
rosalpinx fluid is highly embryotoxic. (C) 1997 by American Society fo
r Reproductive Medicine.