DETRIMENTAL EFFECT OF HYDROSALPINX FLUID ON THE DEVELOPMENT AND BLASTULATION OF MOUSE EMBRYOS IN-VITRO

Objective: To study the effects of human hydrosalpinx fluid on mouse e mbryo blastulation rate. Design: Comparison of mouse embryo blastulati on rate in media containing increasing concentrations of hydrosalpinx fluid. Setting: Tertiary care center. Patient(s): Women undergoing lap aroscopic evaluation or treatment for infertility noted to have hydros alpinx or paratubal cyst. Intervention(s): Exposure of mouse embryos t o hydrosalpinx or paratubal cyst fluid collected during laparoscopy. M ain Outcome Measure(s): Blastulation rate of mouse embryos. Result(s): Culture of mouse embryos at 0% (controls), 0.3%, 0.6%, and 0.9% hydro salpinx fluid concentrations demonstrated significantly lower blastula tion rate at each level compared with the controls. Conclusion(s): Hyd rosalpinx fluid is highly embryotoxic. (C) 1997 by American Society fo r Reproductive Medicine.