ISOLATION OF ACETONE ETHYLENE GLYCOL UTILIZING AND BIOFILM FORMING STRAINS OF BACTERIA/

Packed columns of zeolite A-Ca particles, fed with acetone or/and ethy lene glycol containing synthetic medium, provide a tool for a physiolo gical preparation of bacterial cell populations with solvent-utilizing and biofilm forming activity. Phased procedure and outlined schemes o f column operations enable one to perform (under required conditions) high yield strain isolations and long-term (easy to monitor) physiolog ical adaptations of both activities. Regarding attempts to isolate str ains in the presence of a specific xenobiotic compound, the procedure is ready to be tested/applied across a wider spectrum of xenobiotics. (C) 1997 Elsevier Science B.V.