CELLULASE-CATALYZED GLYCOSYLATION REACTIONS - SIMPLE ROUTE TOWARDS A HIGHLY SELECTIVE SYNTHESIS OF OLIGOSACCHARIDES

Authors
KARTHAUS O SHODA S TAKANO H OBATA K KOBAYASHI S
Citation
O. Karthaus et al., CELLULASE-CATALYZED GLYCOSYLATION REACTIONS - SIMPLE ROUTE TOWARDS A HIGHLY SELECTIVE SYNTHESIS OF OLIGOSACCHARIDES, Journal of the Chemical Society. Perkin transactions. I, (13), 1994, pp. 1851-1857
Citations number
37
Categorie Soggetti
Chemistry Inorganic & Nuclear
Journal title
Journal of the Chemical Society. Perkin transactions. I → ACNP
ISSN journal
0300922X
Issue
13
Year of publication
1994
Pages
1851 - 1857
Database
ISI
SICI code
0300-922X(1994):13<1851:CGR-SR>2.0.ZU;2-3
Abstract
Cellulase (EC 3.2.1.4) can be used for the enzyme-catalysed synthesis of oligosaccharides in a mixed buffer/organic solvent system. Besides glucopyranosides and cellooligosaccharides, non-glucosidic saccharides and modified glucoses as glycosyl accepters also react with beta-lact osyl fluoride as glycosyl donor. The reaction proceeds in a completely stereo- and regio-specific manner, giving rise to coupling products h aving beta (1-->4) glycosidic bonds exclusively. Based on these result s, a model for the active site of cellulase (Trichoderma viride) and t he orientation of the donor and acceptor therein is proposed.