Nk. Smith et al., STATISTICAL OPTIMIZATION OF ENZYMATIC-SYNTHESIS OF DERIVATIVES OF TREHALOSE AND SUCROSE, Enzyme and microbial technology, 21(5), 1997, pp. 349-354
The synthesis of the oligosaccharides 6-O-alpha-mannosyltrehalose, 6-O
-alpha-galactosyltrehalose, 6-O-alpha-galactosyltrehalose, and 6-O-g-a
lpha-mannosylsucrose, using appropriate glycosidases was optimized usi
ng response surface methodology.(1) An empirical mathematical model wa
s constructed to predict the amount of product formed under various co
nditions of pH, substrate concentration, and donor:acceptor ratio. (C)
1997 Elsevier Science Inc.