STATISTICAL OPTIMIZATION OF ENZYMATIC-SYNTHESIS OF DERIVATIVES OF TREHALOSE AND SUCROSE

Citation
Nk. Smith et al., STATISTICAL OPTIMIZATION OF ENZYMATIC-SYNTHESIS OF DERIVATIVES OF TREHALOSE AND SUCROSE, Enzyme and microbial technology, 21(5), 1997, pp. 349-354
Citations number
9
Categorie Soggetti
Biothechnology & Applied Migrobiology
ISSN journal
01410229
Volume
21
Issue
5
Year of publication
1997
Pages
349 - 354
Database
ISI
SICI code
0141-0229(1997)21:5<349:SOOEOD>2.0.ZU;2-B
Abstract
The synthesis of the oligosaccharides 6-O-alpha-mannosyltrehalose, 6-O -alpha-galactosyltrehalose, 6-O-alpha-galactosyltrehalose, and 6-O-g-a lpha-mannosylsucrose, using appropriate glycosidases was optimized usi ng response surface methodology.(1) An empirical mathematical model wa s constructed to predict the amount of product formed under various co nditions of pH, substrate concentration, and donor:acceptor ratio. (C) 1997 Elsevier Science Inc.