RETENTION OF BENZENE-DERIVATIVES ON THE AMINOPROPYL STATIONARY-PHASE IN HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY

The retention of benzene derivatives in the normal-phase mode of high- performance Liquid chromatography (HPLC) using a binary mobile phase ( n-hexane-THF) on aminopropyl silica gel was considered. The possibilit y of describing the retention of the sorbates by known models was eval uated. It was established that molecular interactions between a sorbat e and components of the mobile phase in solution can significantly aff ect the retention of the sorbates and the character of the dependence of the retention on the concentration of a modifier in the mobile phas e. A quantitative description of this dependence was proposed.