EVIDENCE FOR DIRECT CELLULAR PROTECTIVE EFFECT OF PL-10 SUBSTANCES (SYNTHESIZED PARTS OF BODY PROTECTION COMPOUND, BPC) AND THEIR SPECIFICITY TO GASTRIC-MUCOSAL CELLS

The direct gastric mucosal cellular effect of four PL-10 substances (a synthesized part of human body protection compound, BPC containing 14 or 15 amino acids) was studied on freshly isolated rat gastric mucosa l cells and on a mouse myeloma cell line (Sp2/0-Ag14) in an ethanol-in duced cell injury model. The examined substances were not toxic for th e cells. Two of them proved to be significantly protective against the direct cellular damaging effect of ethanol (PL 10.1.15AK-3 in 5 mu g/ ml dose and PL 10.1.AK14-2 dose-dependently, ED50=50 ng/ml) on gastric mucosal cells. This cytoprotective effect was failured on mouse myelo ma cells. Based on these results a part of the in vivo protection indu ced by BPC seems to be a direct cellular protective effect to gastric mucosal cells. (C) 1997 Elsevier Science Inc.