MAPPING OF RAT CHROMOSOME-2 MARKERS GENERATED FROM CHROMOSOME-SORTED DNA

A quantitative trait locus (QTL) for blood pressure has recently been mapped to a region of roughly 30 cM on rat Chromosome (Chr) 2 by linka ge and by the use of congenic strains. For further fine mapping of the QTL, however, closely linked chromosome markers residing in this 30-c M region are required. In the current work, 36 new markers were genera ted by screening rat Chr 2-sorted DNA libraries and subsequently mappe d using five F-2 populations. Combining new and existing markers, the marker density for the 30-cM region approaches, on average, one marker per 1.1 cM.