S. Godard et al., CHARACTERIZATION, GENETIC AND PHYSICAL MAPPING ANALYSIS OF 36 HORSE PLASMID AND COSMID-DERIVED MICROSATELLITES, Mammalian genome, 8(10), 1997, pp. 745-750
Thirty-six new horse microsatellites (II from plasmid libraries and 25
from a cosmid library) were isolated and characterized on a panel of
four horse breeds. Thirty were found to be polymorphic with heterozygo
sity levels ranging between 0.20 and 0.87. Twenty-two of the cosmids w
ere physically mapped to R-banded single horse Chromosomes (Chrs) 1, 3
, 4, 9, 11, 12, 13, 15, 18, 19, 21, 22, 23 and three to pericentromeri
c regions. Furthermore, linkage analysis between a selection of 42 DNA
markers, including those presented in this study, and 16 conventional
markers of the horse hemotype was performed on six paternal half-sib
horse families. Five linkage groups were detected, of which four were
assigned to Chr 10, 11, 15, and 18. This work increased by one-third t
he number of published polymorphic DNA markers suitable for horse mapp
ing and approximately doubled the number of known linkage groups. Our
cosmids labeled 14 out of the 31 horse autosomes. Moreover, the physic
al anchoring of part of these markers will orient linkage and synteny
groups on the chromosomes and will contribute to their assignment.