AUTOMATED ON-CAPILLARY ISOTACHOPHORETIC REACTION CELL FOR FLUORESCENCE DERIVATIZATION OF SMALL SAMPLE VOLUMES AT LOW CONCENTRATIONS FOLLOWED BY CAPILLARY ZONE ELECTROPHORESIS
Nj. Reinhoud et al., AUTOMATED ON-CAPILLARY ISOTACHOPHORETIC REACTION CELL FOR FLUORESCENCE DERIVATIZATION OF SMALL SAMPLE VOLUMES AT LOW CONCENTRATIONS FOLLOWED BY CAPILLARY ZONE ELECTROPHORESIS, Journal of chromatography, 673(2), 1994, pp. 255-266
An automated on-line reaction cell is described combining the concentr
ating properties of isotachophoresis (ITP), the separation efficiency
in capillary zone electrophoresis (CZE) and the detection sensitivity
of laser-induced fluorescence (LIF) detection. The applicability of th
e reaction cell is demonstrated for an automated derivatization proced
ure of glutathion and several amino acids with o-phthaldialdehyde (OPA
) and naphthalene-2,3-dicarboxaldehyde. Nanolitres analyte, at nanomol
ar concentration level, were concentrated and derivatized in capillary
ITP. The derivatives were analyzed by CZE with LIF detection. The ent
ire procedure was automated and took place in the same capillary using
single capillary ITP-CZE. Furthermore, an easy applicable on-capillar
y OPA derivatization is described for CZE without ITP step.