Jp. Jones et al., TRANSCRIPTIONAL REGULATION OF HEXOKINASE-II IN DENERVATED RAT SKELETAL-MUSCLE, Biochemical and biophysical research communications, 238(1), 1997, pp. 53-55
Hexokinase II protein is augmented in denervated skeletal muscle; ther
efore, we determined if hexokinase II gene transcription rates and mRN
A levels are increased with denervation. The right hindlimb skeletal m
uscles of male rats were denervated while the left hindlimbs were sham
operated, Seventy-two h following surgery, rats were sacrificed and t
he gastrocnemius and soleus muscles were harvested for nuclear and RNA
isolation, Nuclear run-on and ribonuclease protection analyses indica
ted th:lt denervation increased hexokinase II transcription rates and
mRNA levels 42% and 88%, respectively (p<0.05). Total hexokinase activ
ity rose 23% in denervated gastrocnemius muscle, In conclusion, the in
crease in hexokinase II gene transcription and mRNA may account for th
e increase in hexokinase II protein and the subsequent rise in total h
exokinase activity in denervated rat skeletal muscle. (C) 1997 Academi
c Press.