K. Hanaki et al., PRIMER TEMPLATE-INDEPENDENT SYNTHESIS OF POLY D(A-T) BY TAQ POLYMERASE/, Biochemical and biophysical research communications, 238(1), 1997, pp. 113-118
Tag DNA polymerase polymerized dATP and dTTP to poly d(A-T) without re
quiring added primer/template in the temperature range of 60-70 degree
s C, Tth DNA polymerase also catalyzed the reaction, while Delta Tth,
Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not
, The reaction was distinct from the template-nonrequiring terminal de
oxynucleotidyl transferase reaction which absolutely required primers.
(C) 1997 Academic Press.