PRIMER TEMPLATE-INDEPENDENT SYNTHESIS OF POLY D(A-T) BY TAQ POLYMERASE/

Authors
HANAKI K ODAWARA T MURAMATSU T KUCHINO Y MASUDA M YAMAMOTO K NOZAKI C MIZUNO K YOSHIKURA H
Citation
K. Hanaki et al., PRIMER TEMPLATE-INDEPENDENT SYNTHESIS OF POLY D(A-T) BY TAQ POLYMERASE/, Biochemical and biophysical research communications, 238(1), 1997, pp. 113-118
Citations number
28
Categorie Soggetti
Biology,Biophysics
Journal title
Biochemical and biophysical research communications → ACNP
ISSN journal
0006291X
Volume
238
Issue
1
Year of publication
1997
Pages
113 - 118
Database
ISI
SICI code
0006-291X(1997)238:1<113:PTSOPD>2.0.ZU;2-R
Abstract
Tag DNA polymerase polymerized dATP and dTTP to poly d(A-T) without re quiring added primer/template in the temperature range of 60-70 degree s C, Tth DNA polymerase also catalyzed the reaction, while Delta Tth, Vent, Vent(exo-), Pfu, Ultma, BcaBEST, and KOD DNA polymerases did not , The reaction was distinct from the template-nonrequiring terminal de oxynucleotidyl transferase reaction which absolutely required primers. (C) 1997 Academic Press.