THE USE OF INHIBITORS OF N-LINKED GLYCOSYLATION AND OLIGOSACCHARIDE PROCESSING TO PRODUCE MONOCLONAL-ANTIBODIES AGAINST NON-PHOSPHORYLCHOLINE EPITOPES OF BRUGIA-PAHANGI EXCRETORY-SECRETORY PRODUCTS

Adult Brugia pahangi were cultured with [H-3]-choline in both the abse nce and the presence of either tunicamycin or 1-deoxymannojirimycin (d MM), inhibitors of N-linked glycosylation and N-linked oligosaccharide processing, respectively. Excretory-secretory products (ES) were reco vered from the spent medium and examined by sodium dodecyl sulfate-pol yacrylamide gel electrophoresis/fluorography. Both inhibitors were fou nd to prevent radiolabeling of ES, a result consistent with blockage o f the addition of the highly immunodominant phosphorylcholine (PC) gro up. Tunicamycin/dMM-treated ES were subsequently employed to immunise a mouse in an attempt to produce monoclonal antibodies (mAbs) against non-PC epitopes of ES. Three mAbs were isolated, each of which reacted with ES but not with PC.