INFLUENCE OF ALKALI-METAL CHLORIDES ON THE DISTRIBUTION OF SERUM-ALBUMIN IN AN AQUEOUS-SOLUTION HYDROCARBON SYSTEM

The processes of serum albumin distribution in the aqueous protein sol ution-interface layer-hydrocarbon system in the presence of lithium, s odium, potassium and cesium chlorides were investigated by the radiome tric phase scintillating method. The kinetic curves of the protein tra nsfer from the electrolyte aqueous solution to the interface and into the hydrocarbon phase were obtained. The equilibrium distribution of t he protein in the system was evaluated. The dependences of adsorption rate constants and protein content at the interface and in the organic phase (both for the equilibrium state) on the concentration of alkali metal chlorides in the range of 10(-2) to 1.2 M were determined. The adsorption rate constant did not depend on the electrolyte concentrati on at high chlorite contents. The salt effect manifested itself (due t o the equilibrium shift) in an increase in the protein content at the interface compared to the organic phase. This effect was most striking for LiCl. According to this retention capacity, the investigated alka li metal chlorides can be aligned in the series: LiCl > CsCl > KCl > N aCl. The retention capacity is connected with the strengthening of coa gulation contacts between the elements of the structure in the interfa ce layer at sufficiently high electrolyte concentrations.