CONSUMPTION OF VEGETABLES REDUCES GENETIC-DAMAGE IN HUMANS - FIRST RESULTS OF A HUMAN INTERVENTION TRIAL WITH CAROTENOID-RICH FOODS

A human intervention study with vegetable products has been performed in twenty three healthy, non smoking males aged 27-40. It was the aim of the study to assess whether consumption of vegetables containing di fferent carotenoids could protect against DNA damage and oxidative DNA damage, The subjects consumed their normal diets, but abstained from vegetables high in carotenoids throughout the study period, After a 2 week depletion period, they received daily 330 ml tomato juice with 40 mg lycopene (weeks 3 and 4), 330 ml carrot juice with 22.3 mg beta-ca rotene and 15.7 mg alpha-carotene (weeks 5 and 6), and 10 g dried spin ach powder (in water or milk) with 11.3 mg lutein (weeks 7 and 8), Blo od was collected weekly and DNA damage was detected in peripheral bloo d lymphocytes with the 'COMET' assay, Oxidised DNA bases were detected by including an incubation step with endonuclease III, The supplement ation of the diet with tomato, carrot or spinach products resulted in a significant decrease in endogenous levels of strand breaks in lympho cyte DNA, Oxidative base damage was significantly reduced during the c arrot juice intervention, These findings support the hypothesis that c arotenoid containing plant products exert a cancer-protective effect v ia a decrease in oxidative and other damage to DNA in humans.