The objective of this research is to investigate mass transfer mechani
sm in biofilms under oscillatory flow conditions, which provides valua
ble information for the understanding of self-purification by biofilms
under oscillatory flow conditions like in coastal areas. A mathematic
al model was developed to describe substrate profiles in biofilms unde
r oscillatory flow conditions, which suggested that substrate uptake r
ate by biofilms is proportional to the square root of the Reynolds num
ber. A laboratory-scale channel with a wave generator was prepared, in
which plastic plates were used as substratum for biofilms. Glucose wa
s used as a substrate. Velocity and turbulence profiles near the biofi
lm were measured by a laser Doppler velocimeter. The cycle of the wave
generator was changed and its short-term effect on the substrate upta
ke rate by the biofilm was measured. The substrate uptake rate decreas
ed with the decrease of the Reynolds number of the wave motion accordi
ng to a power law with a coefficient of 0.6; which suggests that subst
rate transport in biofilms under oscillatory flow conditions is carrie
d out by turbulent diffusion caused by oscillatory flow motions over b
iofilms. (C) 1997 IAWQ. Published by Elsevier Science Ltd.