Rj. Whittington et al., DETECTION OF ANTIBODIES AGAINST IRIDOVIRUSES IN THE SERUM OF THE AMPHIBIAN BUFO-MARINUS, Journal of virological methods, 68(1), 1997, pp. 105-108
Citations number
15
Categorie Soggetti
Virology,"Biochemical Research Methods","Biothechnology & Applied Migrobiology
Sera from the amphibian Bufo marinus (cane or marine toad) were invest
igated using a newly-developed enzyme-linked immunosorbent assay for t
he detection of antibodies to ranaviruses (Family Iridoviridae). Epizo
otic haematopoietic necrosis virus (EHNV) or Bohle iridovirus (BTV) wa
s affinity purified from cell culture supernatants and simultaneously
bound to the solid phase using specific linker antibodies. After bindi
ng to antigen, antibodies in Bufo marinus serum were then detected wit
h a specific anti-immunoglobulin reagent. Of 21 Bufo marinus sera, 3 c
ontained antibodies against EHNV, BIV or related viruses in the ranavi
rus group, at titres greater than 3200. Reactivity of cane toad antibo
dies against BIV, an amphibian virus, was greater than that against si
milar concentrations of EHNV, a piscine virus. (C) 1997 Elsevier Scien
ce B.V.