CLONING OF THE CDNA FOR A NEW MEMBER OF THE IMMUNOGLOBULIN SUPERFAMILY (ISLR) CONTAINING LEUCINE-RICH REPEAT (LRR)

We isolated cDNAs for a novel protein with a calculated molecular mass of 46 kDa, containing a leucine-rich repeat (LRR) with conserved flan king sequences and a C2-type immunoglobulin (Ig)-like domain. This nov el protein was considered to be a new member of the Ig superfamily and was named ISLR (immunoglobulin superfamily containing LRR). These dom ains are known to be important for protein-protein interaction or cell adhesion, and therefore it is possible that the novel protein ISLR ma y also interact with other proteins or cells. Northern blot analysis s howed the presence of a 2.4-kb transcript in various human tissues inc luding retina, heart, skeletal muscle, prostate, ovary, small intestin e, thyroid, adrenal cortex, testis, stomach, and spinal cord as well a s fetal lung and fetal kidney. The ISLR gene was mapped on human chrom osome 15q23-q24 by fluorescence in situ hybridization. (C) 1997 Academ ic Press.