COMPLETION OF THE DNA-SEQUENCE OF MOUSE ADENOVIRUS TYPE-1 - SEQUENCE OF E2B, L1, AND L2 (18-51 MAP UNITS)

The DNA sequence of 9991 nt, corresponding to 18-51 map units of mouse adenovirus type 1 (MAV-1), was determined, completing the sequence of the Larsen strain of MAV-1. The length of the complete MAV-1 genome i s 30946 nucleotides, consistent with previous experimental estimates. The 18-51 map unit region encodes early region 2B proteins necessary f or adenoviral replication as well as late region L1 and L2 structural and packaging proteins. Sequence comparison in this region with human adenoviruses indicates broad similarities, including colinear preserva tion of all recognized open reading frames (ORFs), with highest amino acid identity occurring in the DNA polymerase and polypeptide III (pen ton base subunit) ORFs. Virus-associated (VA) RNA is not encoded in th e region where VA RNAs are found in the human adenoviruses, between E2 B and L1, nor is it encoded anywhere in the entire MAV-1 genome. The M AV-1 polypeptide III lacks the arginine-glycine-aspartic acid (RGD) mo tif which is involved in an association with cell-surface integrins. O nly one RGD sequence is found in an identified coding region in the en tire MAV-1 genome, Similar to the porcine adenovirus, this RGD sequenc e is found in the C-terminus of the MAV-1 fiber protein. (C) 1997 Else vier Science B.V.