CO2 AVAILABILITY AFFECTS ELEMENTAL COMPOSITION (C-N-P) OF THE MARINE DIATOM SKELETONEMA-COSTATUM

The effect of variable CO2 concentrations on the elemental composition (C:N:P) of marine diatoms was investigated in 2 strains of Skeletonem a costatum (Grev.) Cleve. Five or 6 concentrations of dissolved molecu lar carbon dioxide [CO2 (aq)], ranging from 0.5 to 39 mu mol l(-1), we re applied in dilute batch cultures. In both strains, elemental ratios were clearly dependent on [CO2 (aq)]. With decreasing CO2 concentrati ons, a decline in C:P and N:P and an increase in C:N was observed. The close correlation between C:P or N:P and [CO2 (aq)] corresponded to a ca 45 to 65% decrease in elemental ratios from highest (greater than or equal to 30 mu mol l(-1)) to lowest (ca 1 mu mol l(-1)) CO2 concent rations. C:N at low [CO2 (aq)] was up to 24% higher than at high [CO2 (aq)]. To date, the elemental composition of marine phytoplankton has been considered to be independent of CO2 availability. If dependency o f the C:N:P ratio on [CO2 (aq)] proves to be a general phenomenon in m arine phytoplankton, changes in the elemental composition may be expec ted in response to the currently observed increase in partial pressure of atmospheric CO2.