Sm. Saadeddin et Sh. Sobki, RELIABILITY AND PRACTICABILITY OF RANDOM-ACCESS CHEMILUMINESCENCE IMMUNOASSAY ANALYSIS, Medical science research, 25(9), 1997, pp. 579-581
In a study of random access chemiluminescence immunoassay analysis, we
evaluated the reliability and the practicability of the Immulite syst
em for free thyroxine (FT4), thyroid-stimulating hormone (TSH), ferrit
in, prostate specific antigen (PSA), C-peptide and intact parathyroid
hormone (PTH). We observed good within-run reproducibility (ranging fr
om 1.9 to 7.5% CV), as well as between-run precision (ranging from 2.3
to 8.0% CV) for 2-3 levels of controls (low, medium and high) of each
analyte. Comparison with our routine methods showed good correlation
for the tested analytes with an r value ranging from 0.940 to 0.996 an
d a slope of regression line ranging from 0.785 to 1.250. The mean sco
res for the evaluation of the installation process, routine operations
, quality control and other various special parameters were in favour
of the random access chemiluminescent system over the routine automate
d and manual methods used in our laboratory. We conclude that the rand
om access chemiluminescence immunoassay system is reliable for the eva
luation of the tested analytes and has many special features that guar
antee user-friendliness and high practicability in a busy laboratory.