RELIABILITY AND PRACTICABILITY OF RANDOM-ACCESS CHEMILUMINESCENCE IMMUNOASSAY ANALYSIS

In a study of random access chemiluminescence immunoassay analysis, we evaluated the reliability and the practicability of the Immulite syst em for free thyroxine (FT4), thyroid-stimulating hormone (TSH), ferrit in, prostate specific antigen (PSA), C-peptide and intact parathyroid hormone (PTH). We observed good within-run reproducibility (ranging fr om 1.9 to 7.5% CV), as well as between-run precision (ranging from 2.3 to 8.0% CV) for 2-3 levels of controls (low, medium and high) of each analyte. Comparison with our routine methods showed good correlation for the tested analytes with an r value ranging from 0.940 to 0.996 an d a slope of regression line ranging from 0.785 to 1.250. The mean sco res for the evaluation of the installation process, routine operations , quality control and other various special parameters were in favour of the random access chemiluminescent system over the routine automate d and manual methods used in our laboratory. We conclude that the rand om access chemiluminescence immunoassay system is reliable for the eva luation of the tested analytes and has many special features that guar antee user-friendliness and high practicability in a busy laboratory.