PROSTAGLANDIN MEASUREMENT IN MOUSE EMBRYOS OBTAINED DURING IN-VIVO AND IN-VITRO DEVELOPMENT

Our aim was to determine whether prostaglandins (PGs) produced by in v itro-derived embryos are altered. We compared the release of prostagla ndin E-1 and E-2 (PGEs) and prostaglandin F-2 alpha (PGF(2 alpha)) by embryos obtained in vivo PGs produced from embryos derived from in vit ro fertilisation (IVF) and developed in vitro. Different embryo stages (two-cell embryos and blastocysts) produced from in vivo and in vitro development were incubated for 1 h in a bathing medium, PGs were meas ured by radioimmunoassay. The two-cell embryos, and the blastocysts ob tained by the in vitro culture, produced less PGE and PGF(2 alpha) tha n the in vivo-derived embryos.