PLANT-REGENERATION FROM IMMATURE SEED-DERIVED CALLUS OF CYPRIPEDIUM-MACRANTHOS SWARTZ VAR. TAIWANIANUM (MASAMUNE) MAEKAWA,F

To obtain basic information for the micropropagation and future breedi ng of endangered Cypripedium species, plant regeneration from immature seed-derived callus of Cypripedium macranthos var, taiwanianum was st udied, Calli, induced from immature seeds on T medium (Tsutsui and Tom ita 1990) supplemented with 200 mg/l yeast extract, 10 g/l sucrose and 2 g/l gellan gum, were used for the plant regeneration test. The rege neration was observed in three lines of callus on media containing 10( -5)M NAA with or without the addition of 10(-6)M BAP, After vernalizat ion, regenerated plants were established.