I. Meier et al., THE TOMATO RBCS3A PROMOTER REQUIRES INTEGRATION INTO THE CHROMATIN FOR CORRECT ORGAN-SPECIFIC REGULATION, FEBS letters, 415(1), 1997, pp. 91-95
In tomato, the RBCS1, RBCS2 and RBCS3A genes, encoding the small subun
it of ribulose-1,5-bisphosphate carboxylase/oxygenase, are expressed i
n leaves and light-grown seedlings, but only RBCS1 and RBCS2 are expre
ssed in developing tomato fruits, The activities of the three promoter
s have been compared in transgenic plants and after transient transfor
mation, Fruit-specific repression of the RBCS3A promoter was observed
in transgenic plants, but not after ballistic transient transformation
, indicating that chromatin integration is necessary for its correct o
rgan-specific regulation, In addition, matrix attachment regions have
been identified in the RBCS1, RBCS2 and RBCS3A promoters, This is the
second case in plants of absence of correct regulation of a plasmid-bo
rne plant promoter and correlating potential nuclear matrix attachment
of the gene, (C) 1997 Federation of European Biochemical Societies.