V. Chague et al., IDENTIFICATION OF RAPD MARKERS LINKED TO A LOCUS INVOLVED IN QUANTITATIVE RESISTANCE TO TYLCV IN TOMATO BY BULKED SEGREGANT ANALYSIS, Theoretical and Applied Genetics, 95(4), 1997, pp. 671-677
In tomato, Bulked Segregant Analysis was used to identify random ampli
fied polymorphic DNA (RAPD) markers linked to a quantitative trait loc
us (QTL) involved in the resistance to the Tomato Yellow Leaf Curl Vir
us. F-4 lines were distributed into two pools, each consisting of the
most resistant and of the most susceptible individuals, respectively.
Both pools were screened using 600 random primers. Four RAPD markers w
ere found to be linked to a QTL responsible for up to 27.7% of the res
istance. These markers, localized in the same linkage group within a d
istance of 17.3 cM, were mapped to chromosome 6 on the tomato RFLP map
.