PHOTOIMMOBILIZATION OF SULFATED HYALURONIC-ACID FOR ANTITHROMBOGENICITY

Anticoagulant polymer sulfated hyaluronic acid was patterned immobiliz ed on a poly(ethylene terephthalate) (PET) film in a specific pattern by photolithography. Hyaluronic acid was sulfated by a sulfur trioxide -pyridine complex. The polymer was coupled with azidoaniline. The deri vatized polymer was cast on a PET film from aqueous solution. After dr ying, the film was photoirradiated in the presence or absence of a pho tomask. The micropatterning was confirmed by staining with a dye, bril liant green. Since the anticoagulant polymer has negative charges, the cationic dye was adsorbed on the regions where the anticoagulant poly mer was immobilized. Platelet adhesion was reduced on the sulfated hya luronic acid-immobilized areas. The immobilized sulfated hyaluronic ac id significantly reduced thrombus formation.