Mycelial culture of Pleurotus florida produced highest extracellular l
accase in optimum growth medium. At least two laccases (L-1 and L-2) w
ere shown to be present in the culture filtrate. Low-laccase-yielding
mutants with impaired L-2 activity had poor mycelial growth and could
not form fruit body, whereas the revertants from the same mutants were
similar to the parent in mycelial growth and fruit body formation.