PH AND CONCENTRATIONS OF PEPSIN AND TRYPSIN IN FEEDING TUBE ASPIRATESAS PREDICTORS OF TUBE PLACEMENT

Background: The purpose of this study was to determine the extent to w hich pepsin and trypsin concentrations in feeding tube aspirates, in a ddition to pH, contribute to predicting feeding tube position. Methods : Aspirates from 742 feeding tubes (nasogastric, n = 343; nasointestin al, n = 399) were tested for pH and enzyme concentrations. Also tested were aspirates from two feeding tubes inadvertently positioned into t he lung (one in the pleural space and one in the tracheobronchial tree ) and 146 samples of tracheobronchial and pleural fluids collected by other methods. Enzyme assays were conducted in a research laboratory. Results of the pH and enzyme tests were compared with radiologic repor ts of tube location. Results: Gastric fluid had a mean low pH (4.06), a high mean pepsin concentration (349.1 mu g/mL), and a low mean tryps in concentration (19.3 mu g/mL). In contrast, intestinal fluid had a m ean high pH (7.40), a high mean trypsin concentration (143.3 mu g/mL), and a low mean pepsin concentration (24.2 mu g/mL). Respiratory sampl es also had a high mean pH (7.89) but contained little or no pepsin or trypsin. Using a logistic regression equation with all three variable s to differentiate between respiratory and gastrointestinal placement, it was possible to correctly classify 100% of the respiratory cases a nd 93.4% of the gastrointestinal cases. Another equation used to diffe rentiate between gastric and intestinal sites was able to classify cor rectly 91.2% of the gastric cases and 91.5% of the intestinal cases. C onclusions: The results clearly indicate that laboratory-determine enz yme concentrations in feeding tube aspirates are helpful in predicting tube location. Thus, it is desirable that inexpensive, simple bedside tests be developed so that they can be used in conjunction with pH me asurements to help predict tube position.