ALCOHOL-OXIDATION ACTIVITY OF WHOLE CELLS OF PICHIA-PASTORIS ENTRAPPED IN HYBRID GELS COMPOSED OF CA-ALGINATE AND ORGANIC SILICATE

Authors
KAWAKAMI K FURUKAWA SY
Citation
K. Kawakami et Sy. Furukawa, ALCOHOL-OXIDATION ACTIVITY OF WHOLE CELLS OF PICHIA-PASTORIS ENTRAPPED IN HYBRID GELS COMPOSED OF CA-ALGINATE AND ORGANIC SILICATE, Applied biochemistry and biotechnology, 67(1-2), 1997, pp. 23-31
Citations number
7
Categorie Soggetti
Biothechnology & Applied Migrobiology",Biology
Journal title
Applied biochemistry and biotechnology → ACNP
ISSN journal
02732289
Volume
67
Issue
1-2
Year of publication
1997
Pages
23 - 31
Database
ISI
SICI code
0273-2289(1997)67:1-2<23:AAOWCO>2.0.ZU;2-U
Abstract
Formation of hybrid gels from an aqueous mixture of alginate and alkox ysilanes has been applied to immobilization of whole cells of Pichia p astoris catalyzing oxidation of benzyl alcohol in organic solvent. The amount of benzaldehyde produced after a prolonged reaction period was 1.2 and 1.8 times greater with the hybrid gels of alginate + silicate and alginate + methyl-substituted silicate, respectively, than with t he alginate single gel. This was ascribed to a facilitated release rat e of aldehyde, which acted as a strong inhibitor against the enzyme al cohol oxidase, from the inside of the cells to organic medium through hydrophobic gel matrix.