STUDY ON GLUCOSE-OXIDASE FERMENTATION COUPLED WITH MEMBRANE DIALYSIS

The repression of glucose oxidase (GOD) biosynthesis by catabolites du ring fermentation can be alleviated through membrane dialysis fermenta tion (MDF). The results show that the volumetric enzyme productivity o f MDF was two times higher than that of the control (fermentation with out dialysis), and its total enzyme activity was increased by 30-50%. The operation conditions of MDF, such as pore size of the membrane, in itiating time for membrane dialysis, and volume of dialysate used, wer e optimized. The content of amino acids and organic acids in the ferme ntation broth and the dialysate in the reservoir were assessed by amin o acid analyzer and ionic chromatography, respectively. The relationsh ip among the contents of pyruvic acid, gluconic acid, and enzyme activ ity during fermentation was analyzed quantitatively. Furthermore, the effect of membrane dialysis technology applied to the low-yield strain was found to be more effective than that applied to the high-yield st rain.