MUTATIONAL ANALYSIS OF THE CODING REGION OF THE UNCOUPLING PROTEIN-2 GENE IN OBESE NIDDM PATIENTS - IMPACT OF A COMMON AMINO-ACID POLYMORPHISM ON JUVENILE AND MATURITY-ONSET FORMS OF OBESITY AND INSULIN-RESISTANCE

Recently, a gene encoding a novel human uncoupling protein, designated UCP2, was discovered. The murine UCP2 was mapped to a region on mouse chromosome 7 which in several models has been shown to be linked to o besity and hyperinsulinaemia. Single strand conformation polymorphism (SSCP) analysis and direct sequencing of the coding region of the UCP2 gene in 35 obese Caucasian NIDDM patients of Danish ancestry revealed one nucleotide substitution, replacing an alanine with a valine at co don 55. The amino acid polymorphism was present in 24 of the 35 (69 %) examined subjects. The allelic frequency of the A/V55 variant was 48. 3 % (95 % CI: 42.5-54.1 %) among 144 subjects with juvenile onset obes ity, 45.6 % (40.5-50.7 %) among 182 subjects randomly selected at the draft board examination, and 45.5 % (37.1-53.9 %) among lean control s ubjects selected from the same study cohort. Within these cohorts ther e were no differences in BMI values at different ages among wild-type carriers and A/V55 carriers. In a population-based sample of 369 young healthy Caucasians the variant showed no association with alterations in BMI, waist-to-hip ratio, fat mass or weight gain during childhood or adolescence. The A/V55 polymorphism was not related to alterations in fasting values of serum insulin and C-peptide or to an impaired ins ulin sensitivity index. We conclude that genetic variability in the hu man UCP2 gene is not a common factor contributing to NIDDM in obese Da nish Caucasian subjects and the common A/V55 amino acid polymorphism o f the gene is not implicated in the pathogenesis of juvenile or maturi ty onset obesity or insulin resistance in Caucasians.